Current Position: Postdoctoral Research Associate
Department of Biological Science
University of Tulsa, Tulsa, OK

Education

  • Ph.D.(2021) Biological Science (Molecular Microbiology), University of Tulsa, Tulsa, OK
  • M.Sc.(2009) Medical Microbiology, Tribhuvan University, Kathmandu, Nepal
  • B.Sc.(2006) Microbiology, Tribhuvan University, Kathmandu, Nepal

Research Interest

Google Scholar

  1. Molecular characterization of plasmids from foodborne pathogens

FIGURE_1

FIGURE 1. Different types of plasmids identified in our study. Circular Figure represents the pangenome analysis for all plasmid sequences from our laboratory. Individual slot (circle) in the figure represent one plasmid sequence. Pangenome analysis for all plasmid sequences was completed with GView server.

  1. Stress response mechanism of foodborne pathogens

Fig_2

FIGURE 2. Screening for aerotolerant Campylobacter strains by incubating in MHB aerobically with agitation. (A) Hyper-aerotolerant C. coli and C. jejuni (circled) strains surviving a 24 h exposure to aerobic growth conditions. (B) Aerotolerant C. coli and C. jejuni (circled) strains surviving a 12 h exposure to aerobic conditions. Aerosensitive (C) C. jejuni and (D) C. coli strains not surviving the 12 h exposure to aerobic conditions. Error bars represent the mean standard error of triplicate experiments.

  1. Molecular detection of foodborne pathogens

  2. Influence of polymicrobial condition on stress response and pathogenicity

FIGURE 3. Aerotolerance of Campylobacter in mono or mixed cultures containing S. aureus strains or cell-free extracts. (A–D), Survival of C. jejuni T1-21 and C. coli HC2-48 in mono and mixed cultures of S. aureus B4-59C and B6-55A incubated at 25 °C and 42 °C with aerobic conditions. (E) Survival of C. jejuni NCTC11168, (F) C. jejuni OD2-67, and (G) C. coli WA3-33 in media containing cell-free extracts of S. aureus at 42 °C with aerobic conditions. The S. aureus cell-free extracts listed in the Fig. 1 legend were used in this experiment. Campylobacter cells grown in non-amended MHB served as a control. Mean values of CFU counts with SD error bars are represented in figures. Horizontal dotted lines in the figures represent the limit of detection for colony counts (LOD, ~ 33 CFU/ml) in these assays. All data points plotted on the LOD line in figure (A–D) and all the bars not exceeding LOD line in figures (E–G) represent the readings for the respective time points with no detectable CFU counts. Two-way ANOVA with repeated measures (Tukey multiple comparison tests) was conducted in GraphPad Prism 9: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Assignments

Table 1. List of Assignments.

Date Descriptive Name Assignment Link
Septmeber 1, 2022 Preparation of Gihub repository and Rmarkdown Assignment_1
Septmeber 9, 2022 Rmarkdown, Themes Assignment_2